Bla g 2 ELISA 2.0 kit-inbio
ELISA 2.0 kit for measuring Blattella germanica allergen, Bla g 2, cockroach.
Antibodies: 7C11/pAb
Standard Range: 100-0.19 ng/mL
Limit of Detection: 0.78ng/mL
Background: OD<0.2 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:
Plate: Pre-coated with anti-Bla g 2 monoclonal antibody 7C11
Vial 1: (white top) Bla g 2 allergen standard
Concentration: 1000 ng/ml
Vial 2: (brown) Rabbit anti Bla g 2 antibody
Vial 3: (blue top) Peroxidase-conjugated Goat Anti-Rabbit
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C
Expiry: See product insert.
Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.
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