DER F 2 ELISA 2.0 KIT-inbio
ELISA 2.0 kit for measuring Dermatophagoides spp. Group 2 allergen, Der f 2, house dust mite.
*Not recommended for allergen quantification in samples that contain multiple house dust mite species
Antibodies: 1D8/7A1
Standard Range: 250-0.49ng/mL
Limit of Detection: 0.98ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents: Plate: Pre-coated with anti-Der p 2 monoclonal antibody 1D8
Vial 1: (white top) Der f 2 allergen standard
Concentration: 2,500ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 7A1
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: See product insert.
Not Provided: Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.
A list of frequently asked questions and troubleshooting guide can be found under the ‘Support’ tab on our website.