Hep3B cells
Catalog #:C0015001
Origin:
Established from the tumor tissue of an 8-year-old black boy
Species:
Homo sapiens
Tissue:
Liver
Morphology:
Epithelial
Properties:
Adherent
Cytogenic data:
Human hypotriploid stemline with near tetraploid side line
Patient:
Male, 8 years of age
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Protein Expression:
Alpha-foetoproteins, albumin, transferrin, fibrinogen, complement (C3), alpha-2 macroglobulins
DNA Profile (STR):
Amelogenin: X; CSF1PO: 8; D13S317: 12,14; D16S539: 10; D5S818: 13; D7S820: 8,10; THO1: 6,7; TPOX: 9; vWA: 17