HepG2 cells

Catalog #:C0015002

Origin:

Established from the tumor tissue of a 15-year-old Argentine boy

Species:

Homo sapiens

Tissue:

Liver

Morphology:

Epithelial

Properties:

Adherent

Cytogenic data:

human hyperdiploid karyotype

Patient:

Male, 15 yrs of age

Medium:

AddexBio-formulated RPMI-1640 Medium(C0004-01) + 10% FBS

Subculture:

1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37°C

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

I

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

Protein Expression:

Express insulin, insulin-like growth factor II (IGF II), 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities; decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone