DER P 1 ELISA 2.0 KIT
ELISA 2.0 kit for measuring D. pteronyssinus allergen, Der p 1.
Antibodies: 5H8/4C1
Standard Range: 100-0.2ng/mL
Limit of Detection: 0.78ng/mL
Background: OD<0.08 at 450nm
Coefficient of Determination: R-squared>0.98
Contents:
Plate: Pre-coated with anti-Der p 1 monoclonal antibody 5H8
Vial 1: (white top) Der p 1 allergen standard
Concentration: 1000ng/ml
Vial 2: (brown) Biotinylated monoclonal antibody 4C1
Vial 3: (blue top) Streptavidin-peroxidase
Bottle 1: Wash buffer, (10x concentrate)
Bottle 2: Assay buffer, (10x concentrate)
Bottle 3: TMB developing substrate
Bottle 4: Stop solution (0.5N sulfuric acid)
Storage: Store at 2-8°C.
Expiry: See product insert.
Not Provided:
Type I ultrapure water or 18.2MΩ de-ionized water
Volumetric measuring equipment (e.g. serological pipette, graduated cylinder)
Clean containers for buffer and reagent preparation
Calibrated single and multi-channel micropipettes and tips
Vortex mixer
Plate reader capable of reading absorbance at 450nm
Analysis software (recommended, but not required)
Notes: The allergen standard is recommended for immunoassay calibration purposes only.