THLE-2 cells

Catalog #:T0015001

Disease:

Normal, SV40 immortalized, Non-tumorigenic in athymic nude mice.

Origin:

THLE-2 cell line was derived from primary normal liver cells by infection with SV40 large T antigen. -

Species:

Homo sapiens

Tissue:

Liver, left lobe

Morphology:

Epithelial;

Properties:

Adherent

Cytogenic data:

Near diploid

Patient:

Adult

Medium:

BEGM + additives without anti-fungal reagents

Subculture:

When confluent using 0.05% trypsin or trypsin/EDTA, subculture at 1:2 to 1:6; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37°C. Flask pre-coating information please refer to datasheet.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO

Biosafety Level:

II

Sterility:

Bacteria: Negative

Yeast: Negative

Mycoplasma: Negative

Pathogens:

HIV: Negative

Hepatitis B: Negative

Hepatitis C: Negative

DNA Profile (STR):

Amelogenin: X,Y

CSF1PO: 11,13

D13S317: 8,12

D16S539: 11,13

D5S818: 11,13

D7S820: 10,12

THO1: 7,9.3

TPOX: 8,11

vWA: 16,17